Host virus interactions: The infection biology of SARS-CoV-2

In close collaboration with the virology department of the university clinic here in Frankfurt we were able to measure the first quantitative proteomics dataset of human cells infected with SARS-CoV-2. We applied multi-layered proteomics to quantify the dynamic changes of protein synthesis, PTMs and total protein levels over the infection course. We analysed the changes to the host cell proteome to identify pathways important for viral replication. By the identification of these pathways we detected vulnerabilities of the viral replication process that can be targeted by already exisiting drugs. Repurposing these drugs we were able to block the proliferation of the virus in vitro and lay a molecular groundwork for future clincal research.

Bojkova, D., Klann, K., Koch, B. et al. Nature 583, 469–472 (2020)
Klann K, Bojkova D et al. Mol Cell 2020. 80 (1) 164-174.e4

Modification profiling Modification profiling
Protein Dynamics Protein Dynamics