Interaction networks: Profiling protein-protein connections of functional subnetworks

Protein-Protein interactions are essential for cellular function. Upon changing environments proteins can immediately change their interaction partners to fulfill new functions or modulate activities. These changes in activity often cannot be detected at the level of total protein amounts, but contain crucial information about cellular processes. To profile these interaction networks we mainly apply the TurboID system, developed by the Ting lab in Stanford. We are applying this powerful tool to examine how protein-protein interaction networks dynamically change upon stress conditions.