Rukmini worked as a PhD student in the lab of Dr Oishee Chakrabarti at the Saha Institute of Nuclear Physics, Kolkata. She submitted her Ph.D. thesis on E3 ligases and their importance in mitochondrial homeostasis in December 2016. Following this, she worked in the lab of Dr Tony Hunter at the Salk Institute, California. She has joined the Dikic group in October 2017 and worked on ADP-ribosylation of ubiquitin in mammalian cells. She finished her projects and moved on in 2023.