Rukmini worked as a PhD student in the lab of Dr Oishee Chakrabarti at the Saha Institute of Nuclear Physics, Kolkata. She submitted her PhD thesis on E3 ligases and their importance in mitochondrial homeostasis in December 2016. Following this, she worked in the lab of Dr Tony Hunter at the Salk Institute, California. She has joined the Dikic lab in October 2017 and is currently working on ADP-ribosylation of ubiquitin in mammalian cells.